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Journal article

Hydrophilic interaction liquid chromatography (HILIC) in proteomics.

Abstract:

In proteomics, nanoflow multidimensional chromatography is now the gold standard for the separation of complex mixtures of peptides as generated by in-solution digestion of whole-cell lysates. Ideally, the different stationary phases used in multidimensional chromatography should provide orthogonal separation characteristics. For this reason, the combination of strong cation exchange chromatography (SCX) and reversed-phase (RP) chromatography is the most widely used combination for the separa...

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Publication status:
Published

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Publisher copy:
10.1007/s00216-008-1865-7

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
Journal:
Analytical and bioanalytical chemistry
Volume:
391
Issue:
1
Pages:
151-159
Publication date:
2008-05-01
DOI:
EISSN:
1618-2650
ISSN:
1618-2642
Language:
English
Keywords:
Pubs id:
pubs:384606
UUID:
uuid:ce29b9fe-d36e-4386-9cc8-fbb806105408
Local pid:
pubs:384606
Source identifiers:
384606
Deposit date:
2013-11-16

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