CASPR2 Autoimmune Antibodies Induce Neuronal Hyperactivity in Human Brain Organoids

Journal article

Gestational transfer of brain‐reactive antibodies is a risk factor for neurodevelopmental disorders. Contactin‐associated protein‐like 2 (CASPR2) is a known target for pathogenic maternal autoantibodies which have been proposed to interfere with fetal neurodevelopment. However, the impact of CASPR2 antibodies on human brain development remains largely unknown. Here, to better understand the neurophysiological changes that occur in the presence of these pathogenic autoantibodies, we cultured unguided human neural organoids for a period of 6‐months in media containing anti‐CASPR2 antibodies. We then performed neurophysiological characterization via whole‐cell patch‐clamp and calcium imaging in acute organoid slices. Our results reveal that CASPR2 antibody exposure increased spontaneous synaptic activity, enhanced the maximal frequency of action potential firing and of spontaneous network activity. These findings are consistent with a state of neuronal hyperexcitability, a phenotype which is observed in several models of neurodevelopmental disorders. Mechanistically, the alterations observed in action potential waveform are in accordance with a role for CASPR2 in the regulation of voltage‐gated potassium channels and a pathological role for CASPR2 autoantibodies in driving neuronal hyperexcitability. image

Authors: Oliveira, AR; Cammarata, G; Seabra, C; Cardoso, AM; Santos, HJ

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Wiley
• Journal: Journal of Neurochemistry
• Volume: 170
• Issue: 2
• Pages: e70388
• Article number: e70388
• Publication date: 2026-02-23
• Acceptance date: 2026-02-03
• DOI: 10.1111/jnc.70388
• EISSN: 1471-4159
• ISSN: 0022-3042
• Language: English
• Keywords: brain organoids; CASPR2; neurodevelopment; maternal autoantibodies; electrophysiology; autism; hyperexcitability