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SpyRing interrogation: analyzing how enzyme resilience can be achieved with phytase and distinct cyclization chemistries

Abstract:

Enzymes catalyze reactions with exceptional selectivity and rate acceleration but are often limited by instability. Towards a generic route to thermo-resilience, we established the SpyRing approach, cyclizing enzymes by sandwiching between SpyTag and SpyCatcher (peptide and protein partners which lock together via a spontaneous isopeptide bond). Here we first investigated the basis for this resilience, comparing alternative reactive peptide/protein pairs we engineered from Gram-positive bacte...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/srep21151

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
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Name:
Department of Biochemistry, University of Oxford
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Name:
Biotechnology and Biological Sciences Research Council
Publisher:
Nature Publishing Group
Journal:
Scientific Reports More from this journal
Volume:
6
Article number:
21151
Publication date:
2016-01-01
DOI:
ISSN:
2045-2322
Pubs id:
pubs:597670
UUID:
uuid:cdc42be1-26d5-438b-8cd3-3cbbd64d78ca
Local pid:
pubs:597670
Source identifiers:
597670
Deposit date:
2016-01-26

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