Journal article
Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil.
- Abstract:
- Brazil experienced a large dengue virus (DENV) epidemic in 2019, highlighting a continuous struggle with effective control and public health preparedness. Using Oxford Nanopore sequencing, we led field and classroom initiatives for the monitoring of DENV in Brazil, generating 227 novel genome sequences of DENV1-2 from 85 municipalities (2015-2019). This equated to an over 50% increase in the number of DENV genomes from Brazil available in public databases. Using both phylogenetic and epidemiological models we retrospectively reconstructed the recent transmission history of DENV1-2. Phylogenetic analysis revealed complex patterns of transmission, with both lineage co-circulation and replacement. We identified two lineages within the DENV2 BR-4 clade, for which we estimated the effective reproduction number and pattern of seasonality. Overall, the surveillance outputs and training initiative described here serve as a proof-of-concept for the utility of real-time portable sequencing for research and local capacity building in the genomic surveillance of emerging viruses.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, 2.4MB, Terms of use)
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- Publisher copy:
- 10.1038/s41467-021-22607-0
Authors
- Publisher:
- Springer Nature
- Journal:
- Nature Communications More from this journal
- Volume:
- 12
- Issue:
- 1
- Article number:
- 2296
- Publication date:
- 2021-04-16
- Acceptance date:
- 2021-03-17
- DOI:
- EISSN:
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2041-1723
- Pmid:
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33863880
- Language:
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English
- Keywords:
-
- Pubs id:
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1174445
- Local pid:
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pubs:1174445
- Deposit date:
-
2021-11-16
Terms of use
- Copyright holder:
- Adelino et al.
- Copyright date:
- 2021
- Rights statement:
- ©2021 The Author(s). Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
- Licence:
- CC Attribution (CC BY)
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