The luminal Ca(2+) chelator, TPEN, inhibits NAADP-induced Ca(2+) release.

Journal article

The regulation of Ca(2+) release by luminal Ca(2+) has been well studied for the ryanodine and IP(3) receptors but has been less clear for the NAADP-regulated channel. In view of conflicting reports, we have re-examined the issue by manipulating luminal Ca(2+) with the membrane-permeant, low affinity Ca(2+) buffer, TPEN, and monitoring NAADP-induced Ca(2+) release in sea urchin egg homogenate. NAADP-induced Ca(2+) release was almost entirely blocked by TPEN (IC(50) 17-25μM) which suppressed t...

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Authors: Morgan, A; Parrington, J; Galione, A

• Publication status: Published
• Journal: Cell calcium
• Volume: 52
• Issue: 6
• Pages: 481-487
• Publication date: 2012-12-01
• DOI: 10.1016/j.ceca.2012.09.001
• EISSN: 1532-1991
• ISSN: 0143-4160
• Language: English
• Keywords: NADP; Ethylenediamines; Sea Urchins; Calcium; Hydrogen-Ion Concentration; Inositol 1,4,5-Trisphosphate Receptors; Ovum; Animals; Calcium Signaling; Chelating Agents