Repair of abasic sites in DNA.

Journal article

Repair of both normal and reduced AP sites is activated by AP endonuclease, which recognizes and cleaves a phosphodiester bond 5' to the AP site. For a short period of time an incised AP site is occupied by poly(ADP-ribose) polymerase and then DNA polymerase beta adds one nucleotide into the repair gap and simultaneously removes the 5'-sugar phosphate. Finally, the DNA ligase III/XRCC1 complex accomplishes repair by sealing disrupted DNA ends. However, long-patch BER pathway, which is involved in the removal of reduced abasic sites, requires further DNA synthesis resulting in strand displacement and the generation of a damage-containing flap that is later removed by the flap endonuclease. Strand-displacement DNA synthesis is accomplished by DNA polymerase delta/epsilon and DNA ligase I restores DNA integrity. DNA synthesis by DNA polymerase delta/epsilon is dependent on proliferating cell nuclear antigen, which also stimulates the DNA ligase I and flap endonuclease. These repair events are supported by multiple protein-protein interactions.

Authors: Dianov, G; Sleeth, K; Dianova, I; Allinson, S

• Publication status: Published
• Journal: Mutation research
• Volume: 531
• Issue: 1-2
• Pages: 157-163
• Publication date: 2003-10-01
• DOI: 10.1016/j.mrfmmm.2003.09.003
• EISSN: 1873-135X
• ISSN: 0027-5107
• Language: English
• Keywords: DNA Repair; Base Sequence; Molecular Sequence Data