Development of a novel enzyme/prodrug combination for gene therapy of cancer: horseradish peroxidase/indole-3-acetic acid.

Journal article

This paper demonstrates the potential for utilizing the plant enzyme, horseradish peroxidase (HRP), in a gene-directed enzyme prodrug therapy context. Human T24 bladder carcinoma cells transfected with a mammalian expression vector containing the HRP cDNA were selectively sensitized to the nontoxic plant hormone, indole-3-acetic acid (IAA). The HRP/IAA-induced cell kill was effective in normoxic and anoxic conditions. The activated drug is a long-lived species able to cross cell membranes, an...

Expand abstract

Authors: Greco, O; Folkes, L; Wardman, P; Tozer, G; Dachs, G

• Journal: Cancer gene therapy
• Volume: 7
• Issue: 11
• Pages: 1414-1420
• Publication date: 2000-11-01
• DOI: 10.1038/sj.cgt.7700258
• EISSN: 1476-5500
• ISSN: 0929-1903
• Language: English
• Keywords: Dose-Response Relationship, Drug; Flow Cytometry; DNA, Complementary; Inhibitory Concentration 50; Humans; Cell Separation; Models, Chemical; Anoxia; Oxygen; Indoleacetic Acids; Horseradish Peroxidase; Fluorescent Antibody Technique; Blotting, Western; Cell Division; Prodrugs; Gene Therapy; Enzymes; Transfection; Tumor Cells, Cultured; Neoplasms; Plasmids; Time Factors