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Assembly intermediates of orthoreovirus captured in the cell

Abstract:

Traditionally, molecular assembly pathways for viruses are inferred from high resolution structures of purified stable intermediates, low resolution images of cell sections and genetic approaches. Here, we directly visualise an unsuspected ‘single shelled’ intermediate for a mammalian orthoreovirus in cryo-preserved infected cells, by cryo-electron tomography of cellular lamellae. Particle classification and averaging yields structures to 5.6 Å resolution, sufficient to identify secondary str...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41467-020-18243-9

Authors


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Role:
Author
ORCID:
0000-0003-4854-590X
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Role:
Author
ORCID:
0000-0003-0220-8844
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Role:
Author
ORCID:
0000-0002-4480-5439
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Name:
Medical Research Council
Grant:
MR/N00065X/1
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Name:
Biotechnology and Biological Sciences Research Council
Grant:
BB/S003339/1
Publisher:
Springer Nature
Journal:
Nature Communications More from this journal
Volume:
11
Article number:
4445
Publication date:
2020-09-07
Acceptance date:
2020-08-08
DOI:
EISSN:
2041-1723
Pmid:
32895380
Language:
English
Keywords:
Pubs id:
1131254
Local pid:
pubs:1131254
Deposit date:
2021-07-14

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