Lack of requirement for cell cooperation in the antibody response to DNP conjugated to levan.

Journal article

The cellular basis of the antibody response to dinitrophenylated levan (DNP-LE) was investigated using a combination of in vivo and in vitro techniques. The results indicated that the induction of the primary response to DNP-LE is independent of the function of macrophages or T cells. However, these cells may regulate the response to DNP-LE (as for other T-independent antigens), as suggested by the prolongation of the response by adjuvants. No evidence of collaboration between carrier (LE)-reactive and hapten (DNP)-reactive B cells in the induction of the response to DNP-LE was obtained (either by inducing tolerance to LE (in vivo or in vitro) or culturing in the presence of excess LE). Thus DNP-LE, like other thymus-independent antigens (e.g., DNP-polymerized flagellin, DNP-Ficoll) may trigger B cells directly. © 1975.

Authors: Desaymard, C; Feldmann, M

• Publication status: Published
• Journal: Cellular immunology
• Volume: 16
• Issue: 1
• Pages: 106-114
• Publication date: 1975-03-01
• DOI: 10.1016/0008-8749(75)90189-6
• EISSN: 1090-2163
• ISSN: 0008-8749
• Language: English
• Keywords: Mice, Inbred AKR; Polysaccharides; Thymectomy; Lymphocyte Depletion; Cells, Cultured; Macrophages; Male; T-Lymphocytes; Mice, Inbred CBA; Corynebacterium; Bordetella pertussis; Female; B-Lymphocytes; Dinitrophenols; Antibody Formation; Mice; Radiation Chimera; Hemolytic Plaque Technique; Animals; Immune Tolerance; Spleen; Immunization; Antilymphocyte Serum; Freund's Adjuvant