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Effective scavenging at cryotemperatures: further increasing the dose tolerance of protein crystals.

Abstract:

The rate of radiation damage to macromolecular crystals at both room temperature and 100 K has previously been shown to be reduced by the use of certain radical scavengers. Here the effects of sodium nitrate, an electron scavenger, are investigated at 100 K. For sodium nitrate at a concentration of 0.5 M in chicken egg-white lysozyme crystals, the dose tolerance is increased by a factor of two as judged from the global damage parameters, and no specific structural damage to the disulfide bond...

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Publication status:
Published

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Publisher copy:
10.1107/s0909049511007163

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Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
Role:
Author
Journal:
Journal of synchrotron radiation
Volume:
18
Issue:
Pt 3
Pages:
346-357
Publication date:
2011-05-05
DOI:
EISSN:
1600-5775
ISSN:
0909-0495
URN:
uuid:ca726ed9-f336-496e-8333-35f5e95c07fc
Source identifiers:
135014
Local pid:
pubs:135014

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