Plasmodium falciparum: a method for the amplification of S antigens and its application to laboratory and field samples.

Journal article

Polymerase chain reaction amplification of several polymorphic genes has been used to study the population biology of Plasmodium falciparum. S antigen is particularly suitable for such studies, but difficulties in the amplification of this gene have precluded its use. Here we describe a simple method for the amplification of S antigen and show why previous attempts may have been unsuccessful. Data are presented from both laboratory and field isolates.

Authors: Kyes, S; Craig, A; Marsh, K; Newbold, C

• Publication status: Published
• Journal: Experimental parasitology
• Volume: 77
• Issue: 4
• Pages: 473-483
• Publication date: 1993-12-01
• DOI: 10.1006/expr.1993.1108
• EISSN: 1090-2449
• ISSN: 0014-4894
• Language: English
• Keywords: Polymerase Chain Reaction; Polymorphism, Genetic; Plasmodium falciparum; DNA, Protozoan; Base Sequence; Molecular Sequence Data; Animals; Antigens, Protozoan