Mechanism of Polycomb recruitment to CpG islands revealed by inherited disease-associated mutation.

Journal article

How the transcription repressing complex Polycomb interacts with transcriptional regulators at housekeeping genes in somatic cells is not well understood. By exploiting a CpG island (CGI) point mutation causing a Mendelian disease, we show that DNA binding of activating transcription factor (TF) determines histone acetylation and nucleosomal depletion commensurate with Polycomb exclusion from the target promoter. Lack of TF binding leads to reversible transcriptional repression imposed by nuc...

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Authors: Caputo, V; Costa, J; Makarona, K; Georgiou, E; Layton, D

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Oxford University Press Publisher's website
• Journal: Human molecular genetics Journal website
• Volume: 22
• Issue: 16
• Pages: 3187-3194
• Publication date: 2013-01-01
• Acceptance date: 2013-04-05
• DOI: 10.1093/hmg/ddt171
• EISSN: 1460-2083
• ISSN: 0964-6906
• Language: English
• Keywords: Nucleosomes; B-Lymphocytes; Promoter Regions, Genetic; Base Sequence; Gene Expression Regulation; Cells, Cultured; Molecular Sequence Data; DNA-Binding Proteins; Activating Transcription Factors; Polycomb-Group Proteins; Point Mutation; Glycosylphosphatidylinositols; Humans; Protein Binding; CpG Islands; Histones; DNA Methylation; Hemoglobinuria, Paroxysmal; Mannosyltransferases; Genes, Essential