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Very early reaction intermediates detected by microsecond time scale kinetics of cytochrome cd1-catalyzed reduction of nitrite.

Abstract:

Paracoccus pantotrophus cytochrome cd(1) is a nitrite reductase found in the periplasm of many denitrifying bacteria. It catalyzes the reduction of nitrite to nitric oxide during the denitrification part of the biological nitrogen cycle. Previous studies of early millisecond intermediates in the nitrite reduction reaction have shown, by comparison with pH 7.0, that at the optimum pH, approximately pH 6, the earliest intermediates were lost in the dead time of the instrument. Access to early t...

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Publication status:
Published

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Publisher copy:
10.1074/jbc.m804493200

Authors


Strampraad, MJ More by this author
de Vries, S More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
Journal:
The Journal of biological chemistry
Volume:
283
Issue:
41
Pages:
27403-27409
Publication date:
2008-10-05
DOI:
EISSN:
1083-351X
ISSN:
0021-9258
URN:
uuid:c7d39d70-368f-4ae6-ac8e-102bb81cf883
Source identifiers:
100227
Local pid:
pubs:100227

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