Patch-clamp characterisation of somatostatin-secreting -cells in intact mouse pancreatic islets.

Journal article

The perforated patch whole-cell configuration of the patch-clamp technique was applied to superficial cells in intact mouse pancreatic islets. Three types of electrical activity were observed corresponding to alpha-, beta- and delta-cells. The delta-cells were electrically active in the presence of glucose but lacked the oscillatory pattern seen in the beta-cells. By contrast, the alpha-cells were electrically silent at high glucose concentrations but action potentials could be elicited by re...

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Authors: Göpel, S; Kanno, T; Barg, S; Rorsman, P

• Publication status: Published
• Journal: Journal of physiology
• Volume: 528
• Issue: Pt 3
• Pages: 497-507
• Publication date: 2000-11-01
• DOI: 10.1111/j.1469-7793.2000.00497.x
• EISSN: 1469-7793
• ISSN: 0022-3751
• Language: English
• Keywords: Potassium Channels; Mice, Inbred Strains; Electric Conductivity; Mice; Islets of Langerhans; Sodium Channels; Shal Potassium Channels; Patch-Clamp Techniques; Ion Channel Gating; Somatostatin-Secreting Cells; Homeostasis; Potassium Channels, Voltage-Gated; Adenosine Triphosphate; Animals; Electrophysiology; Shaw Potassium Channels; Somatostatin