Immunogenicity of a viral model vaccine after different inactivation procedures.

Journal article

Various strategies for the production of safe vaccines have been used. This study compared three different inactivation procedures, i.e. treatment with formaldehyde, beta-propiolactone or UV-light using vesicular stomatitis virus (VSV) as a model antigen. All three inactivation procedures drastically impaired induction of neutralizing IgG responses, confirming previous observations [Bachmann et al. (1993) J Virol 67:3917-3922]. This reduction could be overcome using higher doses for all three...

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Authors: Bachmann, M; Bast, C; Hengartner, H; Zinkernagel, R

• Publication status: Published
• Publisher: Springer-Verlag
• Journal: Medical microbiology and immunology
• Volume: 183
• Issue: 2
• Pages: 95-104
• Publication date: 1994-05-01
• DOI: 10.1007/bf00277160
• EISSN: 1432-1831
• ISSN: 0300-8584
• Language: English
• Keywords: Mice, Inbred BALB C; Propiolactone; Formaldehyde; Vesicular stomatitis Indiana virus; Vaccines, Inactivated; Mice, Inbred C57BL; T-Lymphocytes, Cytotoxic; Antibodies, Viral; Viral Vaccines; Ultraviolet Rays; Animals; Models, Biological; Cytotoxicity, Immunologic; Immunoglobulin G; Neutralization Tests; Mice