Journal article

Isolation and culture of murine bone marrow-derived macrophages for nitric oxide and redox biology

Abstract:: Macrophages are mononuclear phagocytes derived from haematopoietic progenitors that are widely distributed throughout the body. These cells participate in both innate and adaptive immune responses and lie central to the processes of inflammation, development, and homeostasis. Macrophage physiology varies depending on the environment in which they reside and they exhibit rapid functional adaption in response to external stimuli. To study macrophages in vitro, cells are typically cultured ex vivo from the peritoneum or alveoli, or differentiated from myeloid bone marrow progenitor cells to form bone marrow-derived macrophages (BMDMs). BMDMs represent an efficient and cost-effective means of studying macrophage biology. However, the inherent sensitivity of macrophages to biochemical stimuli (such as cytokines, metabolic intermediates, and RNS/ROS) makes it imperative to control experimental conditions rigorously. Therefore, the aim of this study was to establish an optimised and standardised method for the isolation and culture of BMDMs. We used classically activated macrophages isolated from WT and nitric oxide (NO)-deficient mice to develop a standardised culture method, whereby the constituents of the culture media are defined. We then methodically compared our standardised protocol to the most commonly used method of BMDM culture to establish an optimal protocol for the study of nitric oxide (NO)-redox biology and immunometabolism in vitro.

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Bailey, J. D., Shaw, A., McNeill, E., Nicol, T., Diotallevi, M., Chuaiphichai, S., Patel, J., Hale, A., Channon, K. M., & Crabtree, M. (2020). Isolation and culture of murine bone marrow-derived macrophages for nitric oxide and redox biology. Nitric Oxide: Biology and Chemistry, 100-101(1 August 2020), 17–29.

MLA Style

Bailey, JD, et al. “Isolation and Culture of Murine Bone Marrow-Derived Macrophages for Nitric Oxide and Redox Biology.” Nitric Oxide: Biology and Chemistry, vols. 100-101, no. 1 August 2020, 2020, pp. 17–29.

Chicago Style

Bailey, JD, A Shaw, E McNeill, et al. 2020. “Isolation and Culture of Murine Bone Marrow-Derived Macrophages for Nitric Oxide and Redox Biology.” Nitric Oxide: Biology and Chemistry 100-101 (1 August 2020): 17–29.
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Files:: 1-s2.0-S1089860319303349-main.pdf

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Publisher copy:: 10.1016/j.niox.2020.04.005

Authors

+ Bailey, JD More by this author

Institution:: University of Oxford
Division:: MSD
Department:: RDM
Sub department:: RDM Cardiovascular Medicine
Role:: Author

+ Shaw, A More by this author

Institution:: University of Oxford
Division:: MSD
Department:: RDM
Sub department:: RDM Cardiovascular Medicine
Role:: Author

+ McNeill, E More by this author

Institution:: University of Oxford
Division:: MSD
Department:: RDM
Sub department:: RDM Cardiovascular Medicine
Role:: Author

+ Nicol, T More by this author

Institution:: University of Oxford
Division:: MSD
Department:: RDM
Sub department:: RDM Cardiovascular Medicine
Role:: Author

+ Diotallevi, M More by this author

Institution:: University of Oxford
Division:: MSD
Department:: RDM
Sub department:: RDM Cardiovascular Medicine
Role:: Author

More authors...

+ British Heart Foundation More from this funder

Grant:: FS/14/56/31049

Publisher:: Elsevier
Journal:: Nitric Oxide: Biology and Chemistry More from this journal
Volume:: 100-101
Issue:: 1 August 2020
Pages:: 17-29
Publication date:: 2020-04-24
Acceptance date:: 2020-04-20
DOI:: 10.1016/j.niox.2020.04.005
EISSN:: 1089-8611
ISSN:: 1089-8603
Pmid:: 32339668

Language:: English
Keywords:: Nitric oxide

FFR

Macrophage

MCSF

Tetrahydrobiopterin
Pubs id:: 1102259
Local pid:: pubs:1102259
Deposit date:: 2020-05-04
ARK identifier:: ark:/29072/ora_c6689fc2a53f4eff90da3946ec77fc70

Terms of use

Copyright holder:: Elsevier Inc.
Copyright date:: 2020
Rights statement:: © 2020 Published by Elsevier Inc. This article is available under the Creative Commons CC-BY-NC-ND license

Licence:: CC Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND)

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