Isolation of the human chromosomal band Xq28 within somatic cell hybrids by fragile X site breakage.

Journal article

The chromosomal fragile-site mapping to Xq27.3 is associated with a frequent form of mental retardation and is prone to breakage after induced deoxyribonucleotide pool perturbation. The human hypoxanthine phosphoribosyltransferase (HPRT) and glucose-6-phosphate dehydrogenase (G6PD) genes flank the fragile X chromosome site and can be used to monitor integrity of the site in human-hamster somatic cell hybrids deficient in the rodent forms of these activities. After induction of the fragile X s...

Expand abstract

Authors: Warren, S; Knight, S; Peters, J; Stayton, C; Consalez, G

• Journal: Proceedings of the National Academy of Sciences of the United States of America
• Volume: 87
• Issue: 10
• Pages: 3856-3860
• Publication date: 1990-05-01
• DOI: 10.1073/pnas.87.10.3856
• EISSN: 1091-6490
• ISSN: 0027-8424
• Language: English
• Keywords: Cell Survival; Nucleic Acid Hybridization; Sex Chromosome Aberrations; Clone Cells; Cell Line; X Chromosome; Hypoxanthine Phosphoribosyltransferase; Humans; Chromosome Banding; DNA; Animals; Hybrid Cells; Glucosephosphate Dehydrogenase; DNA Probes; Blotting, Southern; Fragile X Syndrome