Caffeine can override the S-M checkpoint in fission yeast.

Journal article

The replication checkpoint (or 'S-M checkpoint') control prevents progression into mitosis when DNA replication is incomplete. Caffeine has been known for some time to have the capacity to override the S-M checkpoint in animal cells. We show here that caffeine also disrupts the S-M checkpoint in the fission yeast Schizosaccharomyces pombe. By contrast, no comparable effects of caffeine on the S. pombe DNA damage checkpoint were seen. S. pombe cells arrested in early S phase and then exposed t...

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Authors: Wang, S; Norbury, C; Harris, A; Toda, T

• Publication status: Published
• Journal: Journal of cell science
• Volume: 112 ( Pt 6)
• Issue: 6
• Pages: 927-937
• Publication date: 1999-03-01
• EISSN: 1477-9137
• ISSN: 0021-9533
• Language: English
• Keywords: Kinetics; Molecular Sequence Data; Cell Cycle; Protein-Serine-Threonine Kinases; Mitosis; Fungal Proteins; Gene Library; Phosphoprotein Phosphatases; Caffeine; Drug Resistance, Microbial; Schizosaccharomyces; Base Sequence; DNA Damage; Genotype; Amino Acid Sequence; Ribonucleotide Reductases; Schizosaccharomyces pombe Proteins; S Phase; Hydroxyurea; Protein Kinases