Journal article
Gas-phase unfolding assay rapidly predicts structure-function relationships in engineered antibodies with tuned flexibilities
- Abstract:
- Human (h)IgG2 monoclonal antibodies (mAbs) are potent agonists due, in part, to their ability to undergo disulfide shuffling within their hinge regions. Herein, we describe a rapid, sensitive, collision-induced unfolding (CIU) assay that possesses a predictive relationship between gas-phase protein unfolding and agonism in hIgG2 variants. Furthermore, our results highlight the significance of hinge engineering in tuning mAb structure-function relationships for the development of future biotherapeutics.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 4.5MB, Terms of use)
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- Publisher copy:
- 10.1038/s41467-026-75137-y
Authors
- Publisher:
- Nature Research
- Journal:
- Nature Communications More from this journal
- Publication date:
- 2026-06-29
- Acceptance date:
- 2026-06-24
- DOI:
- EISSN:
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2041-1723
- ISSN:
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2041-1723
- Language:
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English
- Keywords:
- Pubs id:
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2442574
- Local pid:
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pubs:2442574
- Source identifiers:
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W7166577412
- Deposit date:
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2026-07-09
- ARK identifier:
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- Copyright date:
- 2026
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