Reconstitution and analysis of the multienzyme Escherichia coli RNA degradosome.

Journal article

The Escherichia coli RNA degradosome is a multienzyme assembly that functions in transcript turnover and maturation of structured RNA precursors. We have developed a procedure to reconstitute the RNA degradosome from recombinant components using modular coexpression vectors. The reconstituted assembly can be purified on a scale that has enabled biochemical and biophysical analyses, and we compare the properties of recombinant and cell-extracted RNA degradosomes. We present evidence that auxil...

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Authors: Worrall, J; Górna, M; Crump, N; Phillips, L; Tuck, A

• Publication status: Published
• Journal: Journal of molecular biology
• Volume: 382
• Issue: 4
• Pages: 870-883
• Publication date: 2008-10-01
• DOI: 10.1016/j.jmb.2008.07.059
• EISSN: 1089-8638
• ISSN: 0022-2836
• Language: English
• Keywords: Multienzyme Complexes; Endoribonucleases; DEAD-box RNA Helicases; Recombinant Proteins; Escherichia coli; RNA Helicases; RNA, Bacterial; Genetic Vectors; Nucleic Acid Conformation; Host Factor 1 Protein; Phosphopyruvate Hydratase; RNA Precursors; Escherichia coli Proteins; Polyribonucleotide Nucleotidyltransferase