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Journal article

Hydrogen ion dynamics in human red blood cells.

Abstract:

Our understanding of pH regulation within red blood cells (RBCs) has been inferred mainly from indirect experiments rather than from in situ measurements of intracellular pH (pH(i)). The present work shows that carboxy-SNARF-1, a pH fluorophore, when used with confocal imaging or flow cytometry, reliably reports pH(i) in individual, human RBCs, provided intracellular fluorescence is calibrated using a 'null-point' procedure. Mean pH(i) was 7.25 in CO(2)/HCO(3)(-)-buffered medium and 7.15 in H...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, Physiology Anatomy and Genetics, BHF Centre of Research Excellence
Role:
Author
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Journal:
The Journal of physiology
Volume:
588
Issue:
Pt 24
Pages:
4995-5014
Publication date:
2010-12-05
DOI:
EISSN:
1469-7793
ISSN:
0022-3751
URN:
uuid:c5755e75-d111-4cde-94a6-704791f96fbc
Source identifiers:
106509
Local pid:
pubs:106509

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