Journal article
Hydrogen ion dynamics in human red blood cells.
- Abstract:
-
Our understanding of pH regulation within red blood cells (RBCs) has been inferred mainly from indirect experiments rather than from in situ measurements of intracellular pH (pH(i)). The present work shows that carboxy-SNARF-1, a pH fluorophore, when used with confocal imaging or flow cytometry, reliably reports pH(i) in individual, human RBCs, provided intracellular fluorescence is calibrated using a 'null-point' procedure. Mean pH(i) was 7.25 in CO(2)/HCO(3)(-)-buffered medium and 7.15 in H...
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- Publication status:
- Published
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- Publisher copy:
- 10.1113/jphysiol.2010.197392
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Authors
Bibliographic Details
- Journal:
- Journal of physiology
- Volume:
- 588
- Issue:
- Pt 24
- Pages:
- 4995-5014
- Publication date:
- 2010-12-01
- DOI:
- EISSN:
-
1469-7793
- ISSN:
-
0022-3751
- Source identifiers:
-
106509
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:106509
- UUID:
-
uuid:c5755e75-d111-4cde-94a6-704791f96fbc
- Local pid:
- pubs:106509
- Deposit date:
- 2012-12-19
Terms of use
- Copyright date:
- 2010
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