Sensorless adaptive optics with a laser free spinning disk confocal microscope

Conference item

Specimen induced aberrations can have detrimental effects in all types of high-resolution microscope. In this study, we present a sensorless technique that uses a deformable mirror (DM) to correct aberrations of both the system and sample. Using a laser-free confocal microscope, with patterned disk illumination and detection. The system is based on a commercial confocal module (Clarity, Aurox Ltd., UK) that uses Light Emitting Diode (LED) illumination to obtain optically sectioned 3D images. The results obtained show that the setup was able to correct aberrations of biological samples used in the study. These systems will help researchers working on various biological systems to obtain improved quality images when focussing deep into thick specimens.

Authors: Hussain, SA; Kubo, T; Hall, N; Hampson, K; Phillips, MA

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Society of Photo-optical Instrumentation Engineers
• Journal: Proceedings of SPIE
• Volume: 11248
• Publication date: 2020-02-17
• Acceptance date: 2019-12-02
• Event title: Adaptive Optics and Wavefront Control for Biological Systems VI
• Event location: San Francisco, California, USA
• Event start date: 2020-02-01
• Event end date: 2020-02-06
• DOI: 10.1117/12.2542959
• EISSN: 1996-756X
• ISSN: 0277-786X
• ISBN: 9781510632592
• Language: English
• Keywords: FFR