Expression of Ly-6 alloantigen during differentiation of cytotoxic T cells.

Journal article

We have prepared anti-Ly-6.2 by immunization of (CBA/Ca X A-Thy-1a)F1 with AKR/Crc lymphocytes and used the antiserum to study heterogencity of peripheral T lymphocytes. The Ly-6 alloantigen is found on all activated cytotoxic T cells studied, directed to a variety of different target antigens. The antiserum reveals heterogeneity among the precursors of T killer cells, i.e. allogeneic precursors are Ly-6-, whereas the generation of cytotoxic cells to hapten-modified syngeneic cells and xenogeneic cells is partially abrogated by serum pretreatment. Precursors of some in vivo primed responses are also Ly-6+. These findings indicate that the expression of Ly-6 antigen is, at least, partially related to the stage of T cell activation. The in vitro proliferative response of T cells to allogeneic Ia antigens is substantially reduced by pretreatment with anti-Ly-6.2 serum suggesting that this antiserum may be a useful tool for the study of heterogeneity in the Ly-1+ pool of T cells.

Authors: Horton, M; Beverley, P; Simpson, E

• Journal: European journal of immunology
• Volume: 9
• Issue: 5
• Pages: 345-352
• Publication date: 1979-05-01
• DOI: 10.1002/eji.1830090502
• EISSN: 1521-4141
• ISSN: 0014-2980
• Language: English
• Keywords: Isoantibodies; T-Lymphocytes; Cytotoxicity, Immunologic; Mice, Inbred Strains; Rats; Phenotype; Killer Cells, Natural; Mice; Isoantigens; Animals; Cell Differentiation; Lymphocyte Cooperation; Lymphocyte Culture Test, Mixed; Lymphocyte Activation