Clavulanic acid dehydrogenase: structural and biochemical analysis of the final step in the biosynthesis of the beta-lactamase inhibitor clavulanic acid.

Journal article

The ultimate step in the biosynthesis of the medicinally important beta-lactamase inhibitor clavulanic acid is catalyzed by clavulanic acid dehydrogenase (CAD). CAD is responsible for the NAPDH-dependent reduction of the unstable intermediate clavulanate-9-aldehyde to yield clavulanic acid. Here, we report biochemical and structural studies on CAD. Biophysical analyses demonstrate that CAD exists as dimeric and tetrameric species in solution. The reaction performed by CAD was shown to be reve...

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Authors: MacKenzie, A; Kershaw, N; Hernandez, H; Robinson, C; Schofield, C

• Publication status: Published
• Journal: Biochemistry
• Volume: 46
• Issue: 6
• Pages: 1523-1533
• Publication date: 2007-02-01
• DOI: 10.1021/bi061978x
• EISSN: 1520-4995
• ISSN: 0006-2960
• Language: English
• Keywords: Spectrometry, Mass, Electrospray Ionization; Crystallization; Alcohol Oxidoreductases; Models, Molecular; Cloning, Molecular; Binding Sites; Escherichia coli; Streptomyces; Crystallography, X-Ray; Clavulanic Acid; Protein Structure, Quaternary