The antibody landscapes following AS03 and MF59 adjuvanted H5N1 vaccination

Journal article

Current seasonal and pre-pandemic influenza vaccines induce short-lived predominantly strain-specific and limited heterosubtypic responses. To better understand how vaccine adjuvants AS03 and MF59 may provide improved antibody responses to vaccination, we interrogated serum from subjects who received 2 doses of inactivated monovalent influenza A/Indonesia/05/2005 vaccine with or without AS03 or MF59 using hemagglutinin (HA) microarrays (NCT01317758 and NCT01317745). The arrays were designed to reflect both full-length and globular head HA derived from 17 influenza A subtypes (H1 to H16 and H18) and influenza B strains. We observed significantly increased strain-specific and broad homo- and heterosubtypic antibody responses with both AS03 and MF59 adjuvanted vaccination with AS03 achieving a higher titer and breadth of IgG responses relative to MF59. The adjuvanted vaccine was also associated with the elicitation of stalk-directed antibody. We established good correlation of the array antibody responses to H5 antigens with standard HA inhibition and microneutralization titers

Authors: Goll, JB; Jain, A; Jensen, TL; Assis, R; Nakajima, R

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Nature Research
• Journal: npj Vaccines
• Volume: 7
• Issue: 1
• Pages: 103-103
• Article number: 103
• Publication date: 2022-08-30
• DOI: 10.1038/s41541-022-00524-7
• EISSN: 2059-0105
• ISSN: 2059-0105
• Language: English
• Keywords: Immunogenicity; Virus; Antibody response; Influenza A virus subtype H5N1; Vaccination; Influenza vaccine; Hemagglutinin (influenza); Antibody titer; Titer; Immunology; Virology; Antigen; Adjuvant; Antibody; Medicine