A highly sensitive whole blood natural killer cell assay.

Journal article

The assessment of natural killer cell activity at baseline and the monitoring of this activity during treatment is important in many diseases especially in patients with cancer and AIDS. An optimised and standardised whole blood chromium release assay is described using K562 cells, the standard target erythroleukaemic cell line. The tumour cell lysis observed using whole blood is comparable to that obtained with the standard 4 h lysis assay using peripheral blood mononuclear cells as effector...

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Authors: Bromelow, K; Galea-Lauri, J; O'Brien, M; Souberbielle, B

• Publication status: Published
• Journal: Journal of immunological methods
• Volume: 217
• Issue: 1-2
• Pages: 177-184
• Publication date: 1998-08-01
• DOI: 10.1016/s0022-1759(98)00109-4
• EISSN: 1872-7905
• ISSN: 0022-1759
• Language: English
• Keywords: Cell Separation; Antigens, CD56; Flow Cytometry; Lymphocyte Depletion; Lymphocyte Subsets; Humans; Cytotoxicity Tests, Immunologic; Sensitivity and Specificity; Anticoagulants; Edetic Acid; Glucose; Killer Cells, Natural; K562 Cells; Reproducibility of Results; Citric Acid; Heparin