Prevalence and sequence diversity of a factor required for actin-based motility in natural populations of Burkholderia species.

Journal article

Actin-based motility of the melioidosis pathogen Burkholderia pseudomallei requires BimA. We report a high degree of conservation of bimA in 99 B. pseudomallei isolates from the area of endemicity. A geographically restricted subset of B. pseudomallei isolates harbored a B. mallei-like bimA allele (12.1%), confounding a differential diagnostic test based on amplification of species-specific bimA regions.

Authors: Sitthidet, C; Stevens, J; Chantratita, N; Currie, B; Peacock, S

• Publication status: Published
• Journal: Journal of clinical microbiology
• Volume: 46
• Issue: 7
• Pages: 2418-2422
• Publication date: 2008-07-01
• DOI: 10.1128/jcm.00368-08
• EISSN: 1098-660X
• ISSN: 0095-1137
• Language: English
• Keywords: Amino Acid Sequence; Sequence Alignment; Endemic Diseases; Sequence Analysis, DNA; Polymorphism, Genetic; Microfilament Proteins; Molecular Sequence Data; Animals; Burkholderia pseudomallei; Burkholderia mallei; Humans; Locomotion; Melioidosis; DNA, Bacterial