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Simultaneous tracking of Pseudomonas aeruginosa motility in liquid and at the solid-liquid interface reveals differential roles for the flagellar stators

Abstract:

Bacteria sense chemicals, surfaces, and other cells and move toward some and away from others. Studying how single bacterial cells in a population move requires sophisticated tracking and imaging techniques. We have established quantitative methodology for label-free imaging and tracking of individual bacterial cells simultaneously within the bulk liquid and at solid-liquid interfaces by utilizing the imaging modes of digital holographic microscopy (DHM) in three dimensions (3D), differential...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1128/msystems.00390-19

Authors


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Role:
Author
ORCID:
0000-0001-7756-8444
More by this author
Role:
Author
ORCID:
0000-0003-1889-5183
Publisher:
American Society for Microbiology
Journal:
mSystems More from this journal
Volume:
4
Issue:
5
Article number:
e00390-19
Publication date:
2019-09-24
Acceptance date:
2019-09-01
DOI:
EISSN:
2379-5077
Pmid:
31551402
Language:
English
Keywords:
Pubs id:
1055853
Local pid:
pubs:1055853
Deposit date:
2021-04-28

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