Journal article
eIF2B is a decameric guanine nucleotide exchange factor with a γ2ε2 tetrameric core
- Abstract:
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eIF2B facilitates and controls protein synthesis in eukaryotes by mediating guanine nucleotide exchange on its partner eIF2. We combined mass spectrometry (MS) with chemical cross-linking, surface accessibility measurements and homology modelling to define subunit stoichiometry and interactions within eIF2B and eIF2. Although it is generally accepted that eIF2B is a pentamer of five non-identical subunits (α–ε), here we show that eIF2B is a decamer. MS and cross-linking of eIF2B complexes all...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 1.9MB, Terms of use)
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- Publisher copy:
- 10.1038/ncomms4902
Authors
Bibliographic Details
- Publisher:
- Springer Nature
- Journal:
- Nature Communications More from this journal
- Volume:
- 5
- Article number:
- 3902
- Publication date:
- 2014-05-23
- Acceptance date:
- 2014-04-15
- DOI:
- EISSN:
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2041-1723
Item Description
- Language:
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English
- Keywords:
- Pubs id:
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pubs:466096
- UUID:
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uuid:c18ed2ef-d2e4-41d4-90bb-209ca456e379
- Local pid:
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pubs:466096
- Source identifiers:
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466096
- Deposit date:
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2014-06-17
Terms of use
- Copyright holder:
- Macmillan Publishers Limited
- Copyright date:
- 2014
- Notes:
- © 2014 Macmillan Publishers Limited. All rights reserved. This work is licensed under a Creative Commons Attribution 3.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.
- Licence:
- CC Attribution (CC BY)
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