Journal article
Cell-free DNA TAPS provides multimodal information for early cancer detection
- Abstract:
- Multimodal, genome-wide characterization of epigenetic and genetic information in circulating cell-free DNA (cfDNA) could enable more sensitive early cancer detection, but it is technologically challenging. Recently, we developed TET-assisted pyridine borane sequencing (TAPS), which is a mild, bisulfite-free method for base-resolution direct DNA methylation sequencing. Here, we optimized TAPS for cfDNA (cfTAPS) to provide high-quality and high-depth whole-genome cell-free methylomes. We applied cfTAPS to 85 cfDNA samples from patients with hepatocellular carcinoma (HCC) or pancreatic ductal adenocarcinoma (PDAC) and noncancer controls. From only 10 ng of cfDNA (1 to 3 ml of plasma), we generated the most comprehensive cfDNA methylome to date. We demonstrated that cfTAPS provides multimodal information about cfDNA characteristics, including DNA methylation, tissue of origin, and DNA fragmentation. Integrated analysis of these epigenetic and genetic features enables accurate identification of early HCC and PDAC.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 459.7KB, Terms of use)
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- Publisher copy:
- 10.1126/sciadv.abh0534
Authors
- Publisher:
- American Association for the Advancement of Science
- Journal:
- Science Advances More from this journal
- Volume:
- 7
- Issue:
- 36
- Article number:
- eabh0534
- Publication date:
- 2021-09-01
- Acceptance date:
- 2021-07-14
- DOI:
- EISSN:
-
2375-2548
- Language:
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English
- Keywords:
- Pubs id:
-
1186259
- Local pid:
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pubs:1186259
- Deposit date:
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2021-07-14
- ARK identifier:
Terms of use
- Copyright holder:
- Siejka-Zielińska et al.
- Copyright date:
- 2021
- Rights statement:
- Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- Licence:
- CC Attribution (CC BY)
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