Journal article
Insights from the crystal structure of the chicken CREB3 bZIP suggest that members of the CREB3 subfamily transcription factors may be activated in response to oxidative stress
- Abstract:
- cAMP response element binding Protein 3 (CREB3) is an endoplasmic reticulum (ER) membrane-bound transcription factor, which belongs to the basic leucine zipper (bZIP) superfamily of eukaryotic transcription factors. CREB3 plays a role in the ER-stress induced unfolded protein response (UPR) and is a multifunctional cellular factor implicated in a number of biological processes including cell proliferation and migration, tumor suppression, and immune-related gene expression. To gain structural insights into the transcription factor, we determined the crystal structure of the conserved bZIP domain of chicken CREB3 (chCREB3) to a resolution of 3.95 Å. The X-ray structure provides evidence that chCREB3 can form a stable homodimer. The chCREB3 bZIP has a structured, pre-formed DNA binding region, even in the absence of DNA, a feature that could potentially enhance both the DNA binding specificity and affinity of chCREB3. Significantly, the homodimeric bZIP possesses an intermolecular disulfide bond that connects equivalent cysteine residues of the parallel helices in the leucine zipper region. This disulfide bond in the hydrophobic core of the bZIP may increase the stability of the homodimer under oxidizing conditions. Moreover, sequence alignment of bZIP sequences from chicken, human, and mouse reveals that only members of the CREB3 subfamily contain this cysteine residue, indicating that it could act as a redox-sensor. Taken together, these results suggest that the activity of these transcription factors may be redox-regulated and they may be activated in response to oxidative stress.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 1.1MB, Terms of use)
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- Publisher copy:
- 10.1002/pro.3573
Authors
+ Biotechnology and Biological Sciences Research Council
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- Grant:
- BBS/E/I/00001651
- Publisher:
- Wiley
- Journal:
- Protein Science More from this journal
- Volume:
- 28
- Issue:
- 4
- Pages:
- 779-787
- Publication date:
- 2019-01-17
- Acceptance date:
- 2019-01-14
- DOI:
- EISSN:
-
1469-896X
- ISSN:
-
0961-8368
- Pmid:
-
30653278
- Language:
-
English
- Keywords:
- Pubs id:
-
pubs:965404
- UUID:
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uuid:c040ec99-9d21-4446-b9d4-fa8951e1cb0e
- Local pid:
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pubs:965404
- Deposit date:
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2019-04-03
- ARK identifier:
Terms of use
- Copyright holder:
- Sabaratnam et al
- Copyright date:
- 2019
- Notes:
- © 2019 The Authors. Protein Science published by Wiley Periodicals, Inc. on behalf of The Protein Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
- Licence:
- CC Attribution (CC BY)
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