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Generation, proliferation and expansion of epithelial cells from primary tissue into mucosoid cultures

Subtitle:
WO2019141824 A1

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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Subgroup:
Oxford Ludwig Institute
Role:
Author
Publisher:
World Intellectual Property Organization Publisher's website
Article number:
PCT/EP2019/051287
Publication date:
2019-07-25
Patent status:
Issued/granted
Patent number:
WO2019141824
Pubs id:
1093503
Local pid:
pubs:1093503
Language:
English
Keywords:
Documentation:
Subject of the invention is a method for long-term culture of columnar epithelial cells and the application thereof. The cells in culture survive more than 6 months and they can be expanded due to their regenerative capacity. The epithelial cells are seeded on a thin matrix-coated solid semi-permeable filter and cultivated in an air liquid interface to form a coherent monolayer. The cultivation method allows for the regeneration and expansion of the culture by enzymatic removal of the cells f... Expand documentation

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