Single-channel currents from zona-free mouse eggs.

Journal article

Cell-attached patch recordings were made from zona-free mouse eggs to examine currents at the single-channel level; two types were identified. A channel with a mean conductance of 130 pS showed changes in reversal potential on dilution of the patch pipette solution consistent with a cation channel; the channel current persisted when the only cation in the pipette was K+: it was concluded that this channel was permeable to K+. The binomial distribution was used to describe the proportion of time 0, 1, 2, ..., n channels were open in multichannel recordings of the K+ channel. Bursts of inward currents of about 1 pA, which showed voltage activation and could be recorded in the presence of CsCl and tetraethylammonium (TEA), were identified as Ca2+ channels.

Authors: Bountra, C; Martin, R

• Publication status: Published
• Journal: Quarterly journal of experimental physiology (Cambridge, England)
• Volume: 72
• Issue: 4
• Pages: 483-492
• Publication date: 1987-10-01
• ISSN: 0144-8757
• Language: English
• Keywords: Temperature; Ovum; Ion Channels; Mice, Inbred BALB C; Animals; Microelectrodes; Membrane Potentials; Calcium; Mice; Potassium