Journal article
HLA-E-restricted SARS-CoV-2-specific T cells from convalescent COVID-19 patients suppress virus replication despite HLA class Ia down-regulation
- Abstract:
- Pathogen-specific CD8+ T cell responses restricted by the nonpolymorphic nonclassical class Ib molecule human leukocyte antigen E (HLA-E) are rarely reported in viral infections. The natural HLA-E ligand is a signal peptide derived from classical class Ia HLA molecules that interact with the NKG2/CD94 receptors to regulate natural killer cell functions, but pathogen-derived peptides can also be presented by HLA-E. Here, we describe five peptides from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that elicited HLA-E–restricted CD8+ T cell responses in convalescent patients with coronavirus disease 2019. These T cell responses were identified in the blood at frequencies similar to those reported for classical HLA-Ia–restricted anti–SARS-CoV-2 CD8+ T cells. HLA-E peptide–specific CD8+ T cell clones, which expressed diverse T cell receptors, suppressed SARS-CoV-2 replication in Calu-3 human lung epithelial cells. SARS-CoV-2 infection markedly down-regulated classical HLA class I expression in Calu-3 cells and primary reconstituted human airway epithelial cells, whereas HLA-E expression was not affected, enabling T cell recognition. Thus, HLA-E–restricted T cells could contribute to the control of SARS-CoV-2 infection alongside classical T cells.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Access Document
- Files:
-
-
(Preview, Accepted manuscript, pdf, 4.1MB, Terms of use)
-
- Publisher copy:
- 10.1126/sciimmunol.abl8881
Authors
+ Wellcome Trust
More from this funder
- Grant:
- 215091/Z/18/Z
- Grant:
- 222426/Z/21/Z
- Grant:
- 200838/Z/16/Z
- Publisher:
- American Association for the Advancement of Science
- Journal:
- Science Immunology More from this journal
- Volume:
- 8
- Issue:
- 84
- Article number:
- eabl8881
- Place of publication:
- United States
- Publication date:
- 2023-06-30
- Acceptance date:
- 2023-06-07
- DOI:
- ISSN:
-
2470-9468
- Pmid:
-
37390223
- Language:
-
English
- Keywords:
- Pubs id:
-
1489449
- Local pid:
-
pubs:1489449
- Deposit date:
-
2024-01-26
- ARK identifier:
Terms of use
- Copyright holder:
- Yang et al
- Copyright date:
- 2023
- Rights statement:
- © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
- Notes:
- This is the accepted manuscript version of the article. The final version is available online from American Association for the Advancement of Science at: https://dx.doi.org/10.1126/sciimmunol.abl8881
If you are the owner of this record, you can report an update to it here: Report update to this record