Journal article
Centrioles generate a local pulse of Polo/PLK1 activity to initiate mitotic centrosome assembly
- Abstract:
- Mitotic centrosomes are formed when centrioles start to recruit large amounts of pericentriolar material (PCM) around themselves in preparation for mitosis. This centrosome “maturation” requires the centrioles and also Polo/PLK1 protein kinase. The PCM comprises several hundred proteins and, in Drosophila, Polo cooperates with the conserved centrosome proteins Spd-2/CEP192 and Cnn/CDK5RAP2 to assemble a PCM scaffold around the mother centriole that then recruits other PCM client proteins. We show here that in Drosophila syncytial blastoderm embryos, centrosomal Polo levels rise and fall during the assembly process—peaking, and then starting to decline, even as levels of the PCM scaffold continue to rise and plateau. Experiments and mathematical modelling indicate that a centriolar pulse of Polo activity, potentially generated by the interaction between Polo and its centriole receptor Ana1 (CEP295 in humans), could explain these unexpected scaffold assembly dynamics. We propose that centrioles generate a local pulse of Polo activity prior to mitotic entry to initiate centrosome maturation, explaining why centrioles and Polo/PLK1 are normally essential for this process.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 3.3MB, Terms of use)
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- Publisher copy:
- 10.15252/embj.2022110891
Authors
+ Engineering and Physical Sciences Research Council
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- Funder identifier:
- https://ror.org/0439y7842
- Grant:
- EP/R020205/1
- Publisher:
- EMBO Press
- Journal:
- The EMBO Journal More from this journal
- Volume:
- 41
- Issue:
- 11
- Article number:
- e110891
- Publication date:
- 2022-05-03
- Acceptance date:
- 2022-03-04
- DOI:
- EISSN:
-
1460-2075
- ISSN:
-
0261-4189
- Language:
-
English
- Keywords:
- Pubs id:
-
1251356
- Local pid:
-
pubs:1251356
- Deposit date:
-
2022-04-21
- ARK identifier:
Terms of use
- Copyright holder:
- Wong et al
- Copyright date:
- 2022
- Rights statement:
- © 2022 The Authors. Published under the terms of the CC BY 4.0 license This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
- Licence:
- CC Attribution (CC BY)
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