Expression of the CMRF-35 antigen, a new member of the immunoglobulin gene superfamily, is differentially regulated on leucocytes.

Daish, A; Starling, G; McKenzie, J; Nimmo, J; Jackson, D; Hart, D

Journal article

Expression of the CMRF-35 antigen, a new member of the immunoglobulin gene superfamily, is differentially regulated on leucocytes.

Abstract:: A new monoclonal antibody, CMRF-35, has been generated that recognized a 224 amino acid cell surface protein which is a novel member of the immunoglobulin gene superfamily. The antibody, raised against large granular lymphocytes (LGL), stains LGL, monocytes, macrophages and granulocytes but not platelets or erythrocytes. In addition, a subset of peripheral blood T lymphocytes (26.6 +/- 13.4% CD5+ cells) and B lymphocytes (13.7 +/- 6.8% CD20+ cells) stained with CMRF-35 but tonsil T and B cells were essentially negative. Expression of the CMRF-35 antigen (Ag) on different leucocyte populations was markedly influenced by stimulation of the cells with mitogens and cytokines. Activation of peripheral blood T cells with phytohaemagglutinin (PHA), or phorbol myristate acetate (PMA) and calcium ionophore (CaI) led to a decrease in the proportion of CMRF-35+ T lymphocytes. In contrast, PHA activation of tonsil T lymphocytes resulted in an increase in CMRF-35 Ag expression (47.1 +/- 1.5% CD5 cells at 6 days). An increase in CMRF-35 Ag was also seen on phorbol ester and CaI-activated tonsil B cells. No change in CMRF-35 expression on natural killer (NK) cells occurred following activation with interleukin-2 (IL-2) but the CMRF-35 Ag was down-regulated following Fc receptor stimulation. A moderate increase in CMRF-35 expression occurred during monocyte-macrophage differentiation and the expression of the Ag on monocytes was differentially regulated by interferon-gamma (IFN-gamma). This regulation of the CMRF-35 Ag on the leucocyte surface suggests that the molecule has an important function common to diverse leucocyte types.

Publication status:: Published

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APA Style

Daish, A., Starling, G., McKenzie, J., Nimmo, J., Jackson, D., & Hart, D. (1993). Expression of the CMRF-35 antigen, a new member of the immunoglobulin gene superfamily, is differentially regulated on leucocytes. Immunology, 79(1), 55–63.

MLA Style

Daish, A., et al. “Expression of the CMRF-35 Antigen, a New Member of the Immunoglobulin Gene Superfamily, Is Differentially Regulated on Leucocytes.” Immunology, vol. 79, no. 1, 1993, pp. 55–63.

Chicago Style

Daish, A, G Starling, J McKenzie, J Nimmo, D Jackson, and D Hart. 1993. “Expression of the CMRF-35 Antigen, a New Member of the Immunoglobulin Gene Superfamily, Is Differentially Regulated on Leucocytes.” Immunology 79 (1): 55–63.
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