Construction and characterisation of a candidate HIV-1 subtype C DNA vaccine for South Africa.

Journal article

A candidate DNA vaccine pTHgagC expressing the immunodeficiency virus-1 (HIV-1) gag gene from South African isolate Du422 was constructed and characterised. The isolate was selected on the basis of being the closest to the South African subtype C consensus sequence. Sequence analysis of cytotoxic T lymphocyte (CTL) epitopes showed that HIV subtype C-infected individuals have CTL responses to a number of epitopes present in the vaccine, but also revealed a more limited presence of subtype A- a...

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Authors: van Harmelen, J; Shephard, E; Thomas, R; Hanke, T; Williamson, A

• Publication status: Published
• Journal: Vaccine
• Volume: 21
• Issue: 27-30
• Pages: 4380-4389
• Publication date: 2003-10-01
• DOI: 10.1016/s0264-410x(03)00406-7
• EISSN: 1873-2518
• ISSN: 0264-410X
• Language: English
• Keywords: Humans; Genes, gag; Animals; South Africa; DNA, Complementary; Cytotoxicity Tests, Immunologic; Mice, Inbred BALB C; Vaccines, DNA; Amino Acid Sequence; Molecular Sequence Data; Hela Cells; Mice; T-Lymphocytes, Regulatory; Chromium Radioisotopes; Interferon-gamma; HIV-1; AIDS Vaccines; Immunization