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Journal article

Application of a new pH-sensitive fluoroprobe (carboxy-SNARF-1) for intracellular pH measurement in small, isolated cells.

Abstract:

We report the use of a new pH-sensitive dual-emission fluoroprobe, carboxy-seminaphthorhodafluor-1 (carboxy-SNARF-1) for ratiometric recording of intracellular pH (pHi) in small isolated cells. The method is illustrated with pHi measurement in single type-1 cells (cell diameter approximately 10 microns) isolated from the carotid body of the neonatal rat. Carboxy-SNARF-1 is loaded using bath application of the acetoxymethyl ester. When excited at 540 nm, the fluoroprobe gives strong, inversely...

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Publication status:
Published

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Publisher copy:
10.1007/bf00370705

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Institution:
University of Oxford
Department:
Oxford, MSD, Physiology Anatomy and Genetics
Role:
Author
Journal:
Pflugers Archiv : European journal of physiology
Volume:
417
Issue:
2
Pages:
234-239
Publication date:
1990-10-05
DOI:
EISSN:
1432-2013
ISSN:
0031-6768
URN:
uuid:ba7b451f-8e94-4c0b-ac5b-5364c2aa60fa
Source identifiers:
312272
Local pid:
pubs:312272

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