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Low-speed centrifugation of retroviral vectors absorbed to a particulate substrate: a highly effective means of enhancing retroviral titre.

Abstract:

For many gene therapy applications the effective titre of retroviral vectors is a limiting factor both in vitro and in vivo. Purification and concentration of retrovirus from packaging cell supernatant can overcome this problem. To this end we have investigated a novel procedure which involves complexing retrovirus to a dense and particulate substrate followed by a short low-speed centrifugation. The study reported here uses heat-killed, formaldehyde fixed Staphylococcus aureus (Pansorbin) ab...

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Publication status:
Published

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Publisher copy:
10.1038/sj.gt.3301201

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Journal:
Gene therapy
Volume:
7
Issue:
11
Pages:
914-923
Publication date:
2000-06-05
DOI:
EISSN:
1476-5462
ISSN:
0969-7128
URN:
uuid:b9dec242-c5f3-4d4c-9bd3-eaf6296ef4fa
Source identifiers:
466392
Local pid:
pubs:466392

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