Lung epithelial cell inhibition of cytokine production by peripheral blood mononuclear cells and lung lymphocytes

Conference item

Type 2 cytokines such as IL-5, IL-13 and IL-4 produced by primed type 2 T cells have been shown to be important in the pathogenesis of eosinophilic airway inflammation. They . Factors regulating the state of activation of these cells are incompletely understood. We and others have shown that release of IL-13 by stimulated T cells can be inhibited by epithelial cells. In this studyto used a PBMC based bulk culture system to: 1) determine whether production of other type-2 cytokines is inhibited by co-culture with epithelial cells; 2) compare inhibition of activated PBMC and human lung lymphocytes; and 3) investigate whether specific soluble mediators modified inhibition of IL-13 release. PBMC isolated from blood and lymphocytes isolated from lung tissue were cultured with IL-2 for five days in the absence and presence of A549 and BEAS2B epithelial cells. The cytokines IL-13, IL-5, IL-9 and TNFa were measured in the supernatant of these cells. We also did similar co-culture experiments in the presence of different inhibitors or blocking cytokine antibodies.

Authors: Layzell, S; Southern, A; Pavord, I; Powell, T

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: BMJ Publishing Group
• Host title: British Thoracic Society Winter Meeting 2017
• Journal: British Thoracic Society Winter Meeting 2017
• Publication date: 2017-11-15
• DOI: 10.1136/thoraxjnl-2017-210983.193