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Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome.

Abstract:

BACKGROUND: Phage-encoded serine integrases, such as φC31 integrase, are widely used for genome engineering. Fifteen such integrases have been described but their utility for genome engineering has not been compared in uniform assays. RESULTS: We have compared fifteen serine integrases for their utility for DNA manipulations in mammalian cells after first demonstrating that all were functional in E. coli. Chromosomal recombination reporters were used to show that seven integrases were active ...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1186/1472-6750-13-87

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Publisher:
BioMed Central Ltd. Publisher's website
Journal:
BMC Biotechnology Journal website
Volume:
13
Issue:
1
Pages:
Article: 87
Publication date:
2013-01-01
DOI:
EISSN:
1472-6750
ISSN:
1472-6750
URN:
uuid:b92a48a1-7e9f-4d49-ab9a-c7e936ddd2fe
Source identifiers:
445067
Local pid:
pubs:445067

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