Journal article
Optimal metabolite transport in hollow fibre membrane bioreactors
- Abstract:
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Hollow fibre membrane bioreactors (HFMBs) provide a fast and efficient method for engineering functional tissue for use in medical treatments. Flow is utilised to overcome mass transport limitations by perfusing nutrient-rich culture medium through the fibre lumen, which can then transport along the fibre lumen or across the porous membrane wall. Cells seeded at the outer membrane wall consume the nutrient and subsequently produce waste metabolites, which are transported away through an external extra-capillary space (ECS) along with excess nutrient. We present and investigate a 2D axisymmetric model for fluid flow and solute transport through a single fibre bioreactor configuration, with cells seeded to the external fibre wall. Fluid flow is modelled by steady lubrication and Darcy equations, which are coupled to the solute transport problem modelled by a system of advection-diffusion equations, supplemented with a reaction term to model the cell layer. Our model analysis reveals how spatially varying wall permeability distributions can be utilised to provide uniform nutrient delivery to a spatially uniform, homogeneous cell population. We also reveal how maximising the transmural pressure drop across the membrane wall is the dominant mechanism for waste removal rather than traditional experimental methods of flushing the ECS.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Version of record, pdf, 1.5MB, Terms of use)
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- Publisher copy:
- 10.1017/flo.2024.18
Authors
- Publisher:
- Cambridge University Press
- Journal:
- Flow: Applications of Fluid Mechanics More from this journal
- Volume:
- 4
- Article number:
- E18
- Publication date:
- 2024-06-14
- Acceptance date:
- 2024-06-13
- DOI:
- EISSN:
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2633-4259
- Language:
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English
- Keywords:
- Pubs id:
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2008212
- Local pid:
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pubs:2008212
- Deposit date:
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2024-06-14
Terms of use
- Copyright holder:
- Booth et al.
- Copyright date:
- 2024
- Rights statement:
- © The Author(s), 2024. Published by Cambridge University Press. This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.
- Notes:
- For the purpose of Open Access, the author has applied a CC BY public copyright licence to any Author Accepted Manuscript (AAM) version arising from this submission.
- Licence:
- CC Attribution (CC BY)
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