Demonstration of respiratory pigments in clonal insulin-producing beta-cells.

Journal article

The amounts of cytochromes and flavoproteins in insulin-producing RINm5F-cells were investigated with dual wavelength spectroscopy. The cytochromes a, a3, b and c were present in normal stoichiometric proportions. Whereas each cytochrome occurred in a concentration corresponding to 25-75 mumol/kg protein, the total amount of flavoproteins was equivalent to about 200 mumol/kg protein. In support of the existence of a functional respiratory chain, the cytochromes reacted normally both to additions of oxygen and to the inhibitors antimycin A and amytal.

Authors: Grapengiesser, E; Rorsman, P

• Publication status: Published
• Journal: Biomedica biochimica acta
• Volume: 44
• Issue: 1
• Pages: 49-54
• Publication date: 1985-01-01
• ISSN: 0232-766X
• Language: English
• Keywords: Cytochromes; Mitochondria; Islets of Langerhans; Oxidation-Reduction; Glycolysis; Energy Metabolism; Flavoproteins; Animals