Defective nuclear localization of Hsp70 is associated with dyserythropoiesis and GATA-1 cleavage in myelodysplastic syndromes.

Journal article

Normal human erythroid cell maturation requests the transcription factor GATA-1 and a transient activation of caspase-3, with GATA-1 being protected from caspase-3-mediated cleavage by interaction with the chaperone heat shock protein 70 (Hsp70) in the nucleus. Erythroid cell dysplasia observed in early myelodysplastic syndromes (MDS) involves impairment of differentiation and excess of apoptosis with a burst of caspase activation. Analysis of gene expression in MDS erythroblasts obtained by ...

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Authors: Frisan, E; Vandekerckhove, J; de Thonel, A; Pierre-Eugène, C; Sternberg, A

• Publication status: Published
• Journal: Blood
• Volume: 119
• Issue: 6
• Pages: 1532-1542
• Publication date: 2012-02-01
• DOI: 10.1182/blood-2011-03-343475
• EISSN: 1528-0020
• ISSN: 0006-4971
• Language: English
• Keywords: Green Fluorescent Proteins; Microscopy, Fluorescence; Oligonucleotide Array Sequence Analysis; Middle Aged; Myelodysplastic Syndromes; Aged; Reverse Transcriptase Polymerase Chain Reaction; Aged, 80 and over; Adult; Erythroblasts; Mutation; Male; HSP70 Heat-Shock Proteins; Female; Cells, Cultured; Erythroid Cells; Cell Nucleus; U937 Cells; Gene Expression Profiling; GATA1 Transcription Factor; Immunoblotting; Caspase 3; Humans; Erythropoiesis; Cell Differentiation