WITHDRAWN: Microsecond resolution of enzymatic conformational changes using dark-field microscopy.

Journal article

We report a novel method to detect angular conformational changes of a molecular motor in a manner sensitive enough to achieve acquisition rates with a time resolution of 2.5mus (equivalent to 400,000fps). We show that this method has sufficient sensitivity to resolve the velocity of the F(1)-ATPase gamma-subunit as it travels from one conformational state to another (transition time). Rotation is detected via a gold nanorod attached to the rotating gamma-subunit of an immobilized F(1)-ATPase. Variations in scattered light intensity allow precise measurement of changes in angular position of the rod below the diffraction limit of light.

Authors: Spetzler, D; York, J; Martin, J; Ishmukhametov, R; Frasch, W

• Journal: Methods (San Diego, Calif.)
• Publication date: 2008-06-01
• DOI: 10.1016/j.ymeth.2008.05.004
• EISSN: 1095-9130
• ISSN: 1046-2023
• Language: English