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Quantitative mass imaging of single biological macromolecules

Abstract:

The cellular processes underpinning life are orchestrated by proteins and their interactions. The associated structural and dynamic heterogeneity, despite being key to function, poses a fundamental challenge to existing analytical and structural methodologies. We used interferometric scattering microscopy to quantify the mass of single biomolecules in solution with 2% sequence mass accuracy, up to 19-kilodalton resolution, and 1-kilodalton precision. We resolved oligomeric distributions at hi...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted Manuscript

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Publisher copy:
10.1126/science.aar5839

Authors


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Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry; Physical & Theoretical Chem
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry; Physical & Theoretical Chem
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry; Physical & Theoretical Chem
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry; Physical & Theoretical Chem
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry; Physical & Theoretical Chem
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Funding agency for:
Collier, MP
More from this funder
Funding agency for:
Chandler, SA
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Publisher:
American Association for the Advancement of Science Publisher's website
Journal:
Science Journal website
Volume:
360
Issue:
6387
Pages:
423-427
Publication date:
2018-04-27
Acceptance date:
2018-03-26
DOI:
EISSN:
1095-9203
ISSN:
0036-8075
Pubs id:
pubs:843982
URN:
uri:b6403cfa-56ae-4675-981b-cc77f69e7dc6
UUID:
uuid:b6403cfa-56ae-4675-981b-cc77f69e7dc6
Local pid:
pubs:843982

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