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Quantitative mass imaging of single biological macromolecules

Abstract:

The cellular processes underpinning life are orchestrated by proteins and their interactions. The associated structural and dynamic heterogeneity, despite being key to function, poses a fundamental challenge to existing analytical and structural methodologies. We used interferometric scattering microscopy to quantify the mass of single biomolecules in solution with 2% sequence mass accuracy, up to 19-kilodalton resolution, and 1-kilodalton precision. We resolved oligomeric distributions at hi...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1126/science.aar5839

Authors


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Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry; Physical & Theoretical Chem
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry; Physical & Theoretical Chem
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry; Physical & Theoretical Chem
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry; Physical & Theoretical Chem
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry; Physical & Theoretical Chem
Role:
Author
More from this funder
Name:
European Research Council
Funding agency for:
Kukura, P
Grant:
Starting Investigator Grant (Nanoscope, 337577)
More from this funder
Name:
Engineering and Physical Sciences Research Council
Funding agency for:
Tkachenko, O
Benesch, JLP
Grant:
Studentship
EP/J01835X/1
More from this funder
Name:
St John’s College, Oxford
Funding agency for:
Tkachenko, O
Grant:
Studentship
More from this funder
Name:
Waters Corp
Funding agency for:
Chandler, SA
Benesch, JLP
Grant:
BB/L017067/1
EP/J01835X/1
More from this funder
Name:
Biotechnology and Biological Sciences Research Council
Funding agency for:
Chandler, SA
Benesch, JLP
Grant:
BB/L017067/1
EP/J01835X/1
Publisher:
American Association for the Advancement of Science
Journal:
Science More from this journal
Volume:
360
Issue:
6387
Pages:
423-427
Publication date:
2018-04-27
Acceptance date:
2018-03-26
DOI:
EISSN:
1095-9203
ISSN:
0036-8075
Pubs id:
pubs:843982
UUID:
uuid:b6403cfa-56ae-4675-981b-cc77f69e7dc6
Local pid:
pubs:843982
Source identifiers:
843982
Deposit date:
2018-04-24

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