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Journal article

Genomic diversity and antimicrobial susceptibility of invasive Neisseria meningitidis in South Africa, 2016–2021

Abstract:

Background: Invasive meningococcal isolates in South Africa have in previous years (<2008) been characterized by serogroup B, C, W, and Y lineages over time, with penicillin intermediate resistance (peni) at 6%. We describe the population structure and genomic markers of peni among invasive meningococcal isolates in South Africa, 2016–2021.

Methods: Meningococcal isolates were collected through national, laboratory-based invasive meningococcal disease (IMD) surveillance. Phenotypic antimicrobial susceptibility testing and whole-genome sequencing were performed, and the mechanism of reduced penicillin susceptibility was assessed in silico.

Results: Of 585 IMD cases reported during the study period, culture and PCR-based capsular group was determined for 477/585 (82%); and 241/477 (51%) were sequenced. Predominant serogroups included NmB (210/477; 44%), NmW (116/477; 24%), NmY (96/477; 20%), and NmC (48/477; 10%). Predominant clonal complexes (CC) were CC41/44 in NmB (27/113; 24%), CC11 in NmW (46/56; 82%), CC167 in NmY (23/44; 53%), and CC865 in NmC (9/24; 38%). Peni was detected in 16% (42/262) of isolates, and was due to the presence of a penA mosaic, with the majority harboring penA7penA9, or penA14.

Conclusions: IMD lineages circulating in South Africa were consistent with those circulating prior to 2008; however, peni was higher than previously reported, and occurred in a variety of lineages.

Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1093/infdis/jiae225

Authors

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Role:
Author
ORCID:
0000-0002-6608-9162
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Institution:
University of Oxford
Division:
MPLS
Department:
Biology
Role:
Author


Publisher:
Oxford University Press
Journal:
Journal of Infectious Diseases More from this journal
Volume:
230
Issue:
6
Pages:
e1311-e1321
Publication date:
2024-04-30
Acceptance date:
2024-04-26
DOI:
EISSN:
1537-6613
ISSN:
0022-1899
Pmid:
38687883


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