Non-Crosslinked Hyaluronic Acid Redensity 1<sup>®</sup> Supports Cell Viability, Proliferation, and Collagen Deposition in Early Burn Management

Journal article

Background/Objectives: Burn injuries pose a significant challenge due to tissue damage and impaired healing. Cell-based therapies offer promise by delivering therapeutic cells to the wound site. However, effective cell delivery remains a critical hurdle. This study investigates the potential of non-crosslinked hyaluronic acid (HA) as a simple, versatile carrier for delivering autologous keratinocytes and fibroblasts to treat early burn wounds. Methods: Primary keratinocytes and fibroblasts were isolated from uninjured adult skin. In addition, fibroblasts and adipose stem cells (ASC) from polydactyly and progenitor fibroblasts were used. Non-cross-linked HA Redensity 1® (RD1) solutions of varying concentrations were prepared and applied to various in vitro models. Cell viability, proliferation, migration, and collagen stimulation were assessed using standard assays. Additionally, cells were suspended in Redensity 1 and applied to an in vitro de-epidemalized dermis (DED) wound model to examine cell delivery and tissue reformation. Results: Preliminary data demonstrated the feasibility of using non-cross-linked HA RD1 gel as a cell carrier. RD1 gel enhanced cell viability, retention, migration, and collagen deposition. Histological analysis revealed improved cell adhesion and migration. Conclusions: This study provides valuable insight into the potential of non-cross-linked HA RD1 as a simple and effective delivery vehicle for cell therapies in early burn care. Successful translation of this approach could significantly improve clinical outcomes for burn patients.

Authors: Liao, Z; Chen, X; Brusini, R; Faivre, J; Applegate, LA

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: MDPI
• Journal: Pharmaceutics
• Volume: 18
• Issue: 1
• Pages: 21
• Publication date: 2025-12-23
• Acceptance date: 2025-12-19
• DOI: 10.3390/pharmaceutics18010021
• EISSN: 1999-4923
• ISSN: 1999-4923
• Pmid: 41599128
• Language: English
• Keywords: Regenerative Medicine; Scarring; Burn Wound Healing; Hyaluronic Acid Hydrogels; Biomaterials; Primary Cells; Tissue engineering; Stem Cell Delivery; Wound healing