Direct demonstration of termination signals for RNA polymerase II from the sea urchin H2A histone gene.

Journal article

Previous studies [1,2] suggested but did not prove that the sea urchin H2A histone gene possesses strong transcriptional termination signals close to, but separate from, the 3' processing signals. In this study we have demonstrated by two independent approaches that these sequences elicit authentic transcriptional termination. First we show by nuclear run off analysis that nascent transcription terminates in the immediate 3' flanking region of the H2A gene, in an A-rich region. Second we show...

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Authors: Briggs, D; Jackson, D; Whitelaw, E; Proudfoot, N

• Publication status: Published
• Journal: Nucleic acids research
• Volume: 17
• Issue: 20
• Pages: 8061-8071
• Publication date: 1989-10-01
• DOI: 10.1093/nar/17.20.8061
• EISSN: 1362-4962
• ISSN: 0305-1048
• Language: English
• Keywords: Histones; Rabbits; Molecular Sequence Data; Globins; Sea Urchins; Hela Cells; Chromosome Deletion; Cell Nucleus; Mutation; Transcription, Genetic; Base Sequence; Transfection; Terminator Regions, Genetic; Genes, Regulator; DNA; RNA Polymerase II; Cloning, Molecular; Genes; Animals; RNA, Messenger; Humans