Journal article

Synthesis of alkyne- and azide-modified oligonucleotides and their cyclization by the CuAAC (click) reaction.

Abstract:: The Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reaction has been used to synthesize cyclic mini-DNA duplexes. The reaction is carried out on 5'-alkyne-3'-azide-labeled hairpin loop oligonucleotides and proceeds in high yield under mild conditions in as little as 5 min. The resultant duplexes have very high thermal stability and their CD spectra are characteristic of normal B-DNA.

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APA Style

El-Sagheer, A., & Brown, T. (2008). Synthesis of alkyne- and azide-modified oligonucleotides and their cyclization by the CuAAC (click) reaction. Current Protocols in Nucleic Acid Chemistry / Edited by Serge L. Beaucage ... [Et Al.], Chapter 4(SUPPL. 35), Unit-4.33.

MLA Style

El-Sagheer, A., and T. Brown. “Synthesis of Alkyne- and Azide-Modified Oligonucleotides and Their Cyclization by the CuAAC (Click) Reaction.” Current Protocols in Nucleic Acid Chemistry / Edited by Serge L. Beaucage ... [Et Al.], vol. Chapter 4, no. SUPPL. 35, 2008, pp. Unit-4.33.

Chicago Style

El-Sagheer, A, and T Brown. 2008. “Synthesis of Alkyne- and Azide-Modified Oligonucleotides and Their Cyclization by the CuAAC (Click) Reaction.” Current Protocols in Nucleic Acid Chemistry / Edited by Serge L. Beaucage ... [Et Al.] Chapter 4 (SUPPL. 35): Unit-4.33.
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Publisher copy:: 10.1002/0471142700.nc0433s35

Authors

+ El-Sagheer, A More by this author

Role:: Author

+ Brown, T More by this author

Institution:: University of Oxford
Division:: MPLS
Department:: Chemistry
Sub department:: Organic Chemistry
Role:: Author

Journal:: Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.] More from this journal
Volume:: Chapter 4
Issue:: SUPPL. 35
Pages:: Unit-4.33
Publication date:: 2008-12-01
DOI:: 10.1002/0471142700.nc0433s35
EISSN:: 1934-9289
ISSN:: 1934-9270

Language:: English
Keywords:: DNA

Catalysis

Oligonucleotides

Cyclization

Copper

Nucleic Acid Conformation

Azides

Alkynes
Pubs id:: pubs:400166
UUID:: uuid:b494c07d-15bb-4a8f-9acc-0f423e294bec
Local pid:: pubs:400166
Source identifiers:: 400166
Deposit date:: 2013-11-16

Terms of use

Copyright date:: 2008

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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